Javad Mohammad Moradi; Ali Akbar Khadem; Seyed Ahmad Hosseini; Arash Veshkini; Ali Asadi Alamouti; Abdollah Mohammadi-Sangcheshmeh
Volume 16, Issue 2 , October 2015, , Pages 75-83
Abstract
In vitro maturation (IVM) was carried out in the presence of different concentrations (10, 50, 100 or 200 µM) of α-linolenic acid (ALA). Embryonic cleavage, blastocyst formation following parthenogenetic activation (PA) and in vitro fertilization (IVF), and numbers of total and apoptotic ...
Read More
In vitro maturation (IVM) was carried out in the presence of different concentrations (10, 50, 100 or 200 µM) of α-linolenic acid (ALA). Embryonic cleavage, blastocyst formation following parthenogenetic activation (PA) and in vitro fertilization (IVF), and numbers of total and apoptotic cells in blastocyst were then determine for the 50 μM concentration and compared with the control group. Out data revealed that ALA increased maturation (MII) rate as compared with control group (P<0.05) and oocytes in 200 μM ALA group showed a lower MII rate as compared with the control group. When oocytes treated with 50 μM ALA were subsequently used for PA or IVF, a higher (P<0.05) rate of blastocyst formation was observed and these embryos had a higher total cell number and a lower apoptotic cell number (P<0.05) as compared with the control group. In conclusion, our results show that supplementation of maturation medium with 50 μM ALA had a positive effect on meiotic maturation by increasing the MII rate and this in turn, stimulated blastocyst formation and also improved quality of the yielded blastocysts.
Somayeh Moeinizadeh; Ali Akbar Khadem; Ali Assadi-Alamouti; Ahmad Afzalzadeh
Volume 15, Issue 1 , July 2014, , Pages 31-43
Abstract
The objectives were to investigate the effects of addition of alfalfa hay as an absorbent on fermentationquality and effluent production of corn silage. Three levels of corn forage with dry matter (DM) contentsof 18.3, 21.7 and 25.5 percent were supplemented with 0, 5, 10, and 20 percent alfalfa hay, ...
Read More
The objectives were to investigate the effects of addition of alfalfa hay as an absorbent on fermentationquality and effluent production of corn silage. Three levels of corn forage with dry matter (DM) contentsof 18.3, 21.7 and 25.5 percent were supplemented with 0, 5, 10, and 20 percent alfalfa hay, on as fedbasis, and ensiled in three replicates in polyvinyl chloride mini-silos in a completely randomized design.Concentrations of ash and total nitrogen increased and that of neutral detergent fiber decreasedsignificantly as the level of absorbent increased (P< 0.05). Increasing the level of absorbent significantlyincreased the DM of silage where the higher levels of DM were observed in silages with 10 and 20percent absorbent. The pH values were within the optimum range of corn silage (ranging from 3.79 to4.33); however, silage pH was affected by the interaction between DM and level of absorbent (P<0.05).The pH increased as the level of absorbent increased, however, the effect was less observed in silageswith 26 percent DM. Also, there was an interaction between the DM and absorbent in the concentration ofresidual water-soluble carbohydrate of silage where the differences in water-soluble carbohydrates insilages with 18 percent initial DM were lower than those in silages with 22 and 26 percent DM. As thelevel of absorbent increased, aerobic stability was improved with the effects becoming less pronounced asthe level of DM increased. Effluent production was markedly reduced as the level of absorbent increasedso that it dropped to 0 ml at 20 percent alfalfa hay addition at all DM levels (P<0.05). The concentrationsof DM and nitrogen were higher in effluent produced from silages treated with higher level of absorbent;however, total DM and nitrogen losses were lower due to considerably lower effluent production. Resultsshowed that using a minimum of 10 percent alfalfa hay could effectively reduce effluent production andimprove aerobic stability of corn silage while maintaining its optimal pH.
Golestan Golestani Milanloo; seyed davood sharifi; Akbar Yaghoub far; Ali akbar Khadem
Volume 13, Issue 2 , March 2012, , Pages 1-10
Abstract
This study was carried out to investigate the effects of Natuzyme Plus (a multi enzyme) in diets containing wheat and canola meal on broiler performance. A total of 336 day-old Arbor Acres broiler chicks were used in a completely randomized design with six treatments and four replicates. Experimental ...
Read More
This study was carried out to investigate the effects of Natuzyme Plus (a multi enzyme) in diets containing wheat and canola meal on broiler performance. A total of 336 day-old Arbor Acres broiler chicks were used in a completely randomized design with six treatments and four replicates. Experimental diets were: 1 . Diet based on corn-soybean meal (control), 2 . Diet containing 30 precent wheat and 3 . Diet containing 30 percent wheat + 10 percent canola meal, which Natozyme was added (350 g/ton) to these diets to make diets containing enzyme (totally six treatments). Adding enzyme to diet containing wheat and canola meal increased significantly feed intake, weight gain and feed conversion ratio (FCR) at starter and overall rearing period (P< 0.01). Use of Natuzyme Plus in diet containing wheat and canola meal reduced significantly the relative weight of digestive organs in compared of same diets without enzyme. The results of this study indicate that the performance of broiler chickens fed the diets containing wheat and canola meal could be improve by supplementing with Natuzyme plus preparations.
Behzad Khorrami; Ali Akbar Khadem; Ahmad Afzalzadeh; Mohammad Ali Norouzian
Volume 13, Issue 2 , March 2012, , Pages 29-38
Abstract
In this study, about 1000 kg of wet Rose Flower pulp were obtained from Ghamsar – Kashan in Iran and dried in air for 10 days. The DM, CP, Ash, EE, total extractable phenols, total tannin, Ca and P of Rose flower extraction pulp (RFEP) were 96.0, 12.5, 6.5, 1.8, 2.7, 1.3, 1.1 and 0.1 percent, respectively. ...
Read More
In this study, about 1000 kg of wet Rose Flower pulp were obtained from Ghamsar – Kashan in Iran and dried in air for 10 days. The DM, CP, Ash, EE, total extractable phenols, total tannin, Ca and P of Rose flower extraction pulp (RFEP) were 96.0, 12.5, 6.5, 1.8, 2.7, 1.3, 1.1 and 0.1 percent, respectively. Four adult Chaal sheep in a changeover design used for determination of digestibility, dry matter intake and nitrogen balance. The sheep fed by four diets with different ratio of RFEP: alfalfa (0.0:100, 15:85, 30:70 and 45:55). Digestibility of DM and OM were not affected by experimental diets. Inclusion of 30 percent or more RFEP, decreased CP digestibility (P< 0.05). Dry matter intake was not affected by inclusion of different levels of RFEP in diets. The nitrogen balance of diets including zero, 15, 30 and 45 precent RFEP were 3.1, 2.6, 1.4 and 1.1 gram per day, respectively (P< 0.05). The DM and CP effective degradability of RFEP were 68.5 and 67.8, respectively. It seems that Rose flower extraction pulp can be used as a part of roughage in the ruminant’s diet.
M. A. Alamian; A. A. Khadem; S. D. Sharifi
Volume 14, Issue 2 , January 2012, , Pages 1-10
Abstract
The effects of phytase and glycosidase supplementation to diets containing rice bran on broiler performance were investigated by using 200 day old (308 Ross) broiler chicks in a completely randomized design with five treatments and four replicates. The five dietary treatments were as: corn-soybean meal ...
Read More
The effects of phytase and glycosidase supplementation to diets containing rice bran on broiler performance were investigated by using 200 day old (308 Ross) broiler chicks in a completely randomized design with five treatments and four replicates. The five dietary treatments were as: corn-soybean meal based diet; diet containing rice bran; diet containing rice bran supplemented with Natafous; diet containing rice bran supplemented with Safizyme, and diet containing rice bran supplemented with Natafous and Safizyme. The dietary levels of rice bran were 15, 20 and 25 percent for the starting, growing and finishing diets, respectively. Birds fed on rice bran + enzyme added diets consumed less feed (P< 0.05) than those fed on unsupplemented rice bran contained diets. The enzyme addition to rice bran containing diets improved the villi length and weight gain of birds at different rearing periods (P< 0.05). In conclusion, results showed that the Natafous or Safizyme supplementation in diets could improve the performance of broiler chicks when rice bran was used at a level of 15, 20 and 25 percent in starter, grower and finisher diets, respectively.
Ahmad Afzalzadeh; Mohsen Absalan; seyed davood sharifi; Ali Akbar Khadem; Davoud Ghandi
Volume 13, Issue 1 , September 2011, , Pages 41-48
Abstract
To study the effects of various levels of whole cottonseed (WCS) on performance, carcass characteristics and blood parameters, an experiment in completely randomized design (CRD) with four diets containing zero, four, eight and 16 percent WCS on 20 Zandi male lambs with initial average weight 30.4 ± ...
Read More
To study the effects of various levels of whole cottonseed (WCS) on performance, carcass characteristics and blood parameters, an experiment in completely randomized design (CRD) with four diets containing zero, four, eight and 16 percent WCS on 20 Zandi male lambs with initial average weight 30.4 ± 1.8 kg and 5-6 months old for 90 days was performed. Energy and chemical composition of rations were similar. In comparison of control diet with diets contain WCS in aspect of feed intake, feed conversion ratio, the percent of the carcass and also abdominal fat was significant difference (P< 0.01). The effect of diet on concentration of blood parameters was significant (P< 0.01). Glucose concentration with increasing of WCS in diet was decreasing, whereas concentration of cholesterol and triglyceride in blood increased linearly. This experiment showed that the WCS could be included up to eight percent of the fattening male lamb’s diet.
Seyed Davoud Sharifi; Ehsan Tavazoei; Ali Akbar Khadem; Abbas Barin
Volume 12, Issue 2 , October 2010, , Pages 11-20
Abstract
In this study, the effect of different levels of fat with antibiotics and probiotics on blood biochemical traits and performance of broiler chicks was investigated. In a factorial experiment, 288 Day-old Ross broiler chicks treated with two level of fat (3 and 6 percent) and three additives (Flavomycin, ...
Read More
In this study, the effect of different levels of fat with antibiotics and probiotics on blood biochemical traits and performance of broiler chicks was investigated. In a factorial experiment, 288 Day-old Ross broiler chicks treated with two level of fat (3 and 6 percent) and three additives (Flavomycin, Protexin and none-additive). The birds were allocated randomly in six treatments with four replicates and 12 birds per replicat. Results showed that the chicks fed on diets containing 3 percent of fat + Flavomycin had higher feed intake and body weight gain compared to birds fed with fat + Protexin (P< 0.05). The concentration of cholesterol, HDL and LDL in serum of birds of 6% fat was more than of those fed diets with 3% fat. Birds fed diets containing protexin had lowest cholesterol, HDL and LDL (P< 0.05). It was concluded that supplementing the rations containing fat with probiotics decrease feed intake and body weight gain in broilers chicks and their performance decrease by increasing fat in diets.
Ahmad Afzalzadeh; Hadi Ghorbani Farmad; mohsen danesh mesgaran; Ali Akbar Khadem
Volume 12, Issue 2 , October 2010, , Pages 37-50
Abstract
The effect of feeding of soaked barley straw on milk production and composition and rumen characteristics was studied. The degradability of soaked barley straw (for 24, 48 and 72 hours) and unsoaked barley straws were studied in the first experiment. The unsoaked barley straw had more readily degraded ...
Read More
The effect of feeding of soaked barley straw on milk production and composition and rumen characteristics was studied. The degradability of soaked barley straw (for 24, 48 and 72 hours) and unsoaked barley straws were studied in the first experiment. The unsoaked barley straw had more readily degraded fraction (a) than soaked straw treatments (p> 0.05). There was no significant difference between slowly degradable fractions (b) and degradation rates (c, %h) in various treatments of barley straw. The degradability parameters of different rations also studied. The differences between readily (a) and slowly (b) degradable fractions were not significant. In the second experiment the effect of partial replacement of alfalfa hay with unsoaked barley straw and 24h soaked barley straw on performance of 9 multiparous lactating dairy cows in midlactation (580.9 ± 39.3 BW) with 27.8 ± 4.5 kg daily milk yield was studied in a crossover design. The effects of different treatments on milk yield (p< 0.05), milk fat (g/kg yield) (p< 0.05), N-NH3 rumen liquor (p< 0.0001), rumination and chewing activity (min/d) were significant. The rumination and chewing activity (min/d) were increased in diets that contained barley straw. In conclusion utilization of soaked barley straw in ration of midlactation cows is recommended.